David Noble
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Add detailed citation info
Browse files- README.md +42 -3
- data/YM_0549/README.md +2 -0
README.md
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- config_name: YM_1068
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data_files: "data/YM_1068/data.parquet"
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---
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#
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Protein-protein interactions (PPIs) are fundamental to countless biological processes. One of the most informative biophysical properties of a PPI is the binding affinity: the strength of how two proteins interact. Yet, despite its importance, publicly available affinity data remains limited, constraining the development and benchmarking of protein modeling methods.
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| YM_0989 | Trastuzumab-scFv CDR3 Optimization Variants Iter0 x HER-2 | [details](./data/YM_0989/README.md) |
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| YM_0990 | Trastuzumab-scFv CDR+FW Optimization Variants Iter0 x HER-2 | [details](./data/YM_0990/README.md) |
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## Dataset schema
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| Column | Description |
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### How should I cite this dataset?
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### Can I use this dataset for model training or benchmarking?
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- config_name: YM_1068
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data_files: "data/YM_1068/data.parquet"
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---
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# Open Protein–Protein Interaction Affinity Datasets with AlphaSeq
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Protein-protein interactions (PPIs) are fundamental to countless biological processes. One of the most informative biophysical properties of a PPI is the binding affinity: the strength of how two proteins interact. Yet, despite its importance, publicly available affinity data remains limited, constraining the development and benchmarking of protein modeling methods.
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| YM_0989 | Trastuzumab-scFv CDR3 Optimization Variants Iter0 x HER-2 | [details](./data/YM_0989/README.md) |
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| YM_0990 | Trastuzumab-scFv CDR+FW Optimization Variants Iter0 x HER-2 | [details](./data/YM_0990/README.md) |
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## References
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This dataset collection builds on the AlphaSeq high-throughput yeast mating assay technology and includes data from multiple publications:
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### Core Technology
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- Younger, D., Berger, S., Baker, D., & Klavins, E. (2017). High-throughput characterization of protein-protein interactions by reprogramming yeast mating. Proceedings of the National Academy of Sciences of the United States of America, 114(46), 12166–12171. [https://doi.org/10.1073/pnas.1705867114](https://doi.org/10.1073/pnas.1705867114)
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### Dataset Sources
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**YM_0005:**
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- Engelhart, E., Lopez, R., Emerson, R., Lin, C., Shikany, C., Guion, D., Kelley, M., & Younger, D. (2022). Massively multiplexed affinity characterization of therapeutic antibodies against SARS-CoV-2 variants. Antibody therapeutics, 5(2), 130–137. [https://doi.org/10.1093/abt/tbac011](https://doi.org/10.1093/abt/tbac011)
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**YM_0549, YM_0693, YM_0852, YM_0985, YM_0988, YM_0989, YM_0990, YM_1068:**
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- Agarwal, A. A., Harrang, J., Noble, D., McGowan, K. L., Lange, A. W., Engelhart, E., Lahman, M. C., Adamo, J., Yu, X., Serang, O., Minch, K. J., Wellman, K. Y., Younger, D. A., Lopez, R. M., & Emerson, R. O. (2025). AlphaBind, a domain-specific model to predict and optimize antibody-antigen binding affinity. mAbs, 17(1), 2534626. [https://doi.org/10.1080/19420862.2025.2534626](https://doi.org/10.1080/19420862.2025.2534626)
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## Dataset schema
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| Column | Description |
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### How should I cite this dataset?
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To properly acknowledge this work, please cite:
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1. **This dataset repository:**
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```text
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A-Alpha Bio (2025). Open Protein–Protein Interaction Affinity Datasets with AlphaSeq.
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https://huggingface.co/datasets/aalphabio/open-alphaseq
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```
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2. **The AlphaSeq technology paper:**
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```text
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Younger, D., Berger, S., Baker, D., & Klavins, E. (2017).
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High-throughput characterization of protein–protein interactions by reprogramming yeast mating.
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Proceedings of the National Academy of Sciences, 114(46), 12166-12171.
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https://doi.org/10.1073/pnas.1705867114
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```
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3. **The specific experiment paper(s) for the dataset(s) you use:**
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- **If using YM_0005:** Cite the CoV epitope mapping paper (Engelhart et al., 2022) - see [References](#references) section
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- **If using any other experiments (YM_0549, YM_0693, YM_0852, YM_0985, YM_0988, YM_0989, YM_0990, YM_1068):** Cite the AlphaBind paper (2025) - see [References](#references) section
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For complete citation details and DOI links, see the [References](#references) section above.
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### Can I use this dataset for model training or benchmarking?
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data/YM_0549/README.md
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This dataset includes 29765 unique VHHs and 8 unique RBD sequences. The alpha-library is the same as YM_1068.
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## Misc dataset details
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We define the following binders:
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This dataset includes 29765 unique VHHs and 8 unique RBD sequences. The alpha-library is the same as YM_1068.
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A more extensive methods section can be found in our publication [here](https://pmc.ncbi.nlm.nih.gov/articles/PMC12296056/).
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## Misc dataset details
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We define the following binders:
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