add geo GSE311976

.gitattributes

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 脓毒症/转录组/arrayexpress_E-MTAB-15687/nv_rma_expression_matrix_anno.txt filter=lfs diff=lfs merge=lfs -text
 脓毒症/转录组/geo_GSE283237/GSE283237_normalized_count_table.xlsx filter=lfs diff=lfs merge=lfs -text
 脓毒症/转录组/geo_GSE283237/GSE283237_TPM_table.xlsx filter=lfs diff=lfs merge=lfs -text
+脓毒症/转录组/geo_GSE311976/GSE311976_1_genes_fpkm_expression.xlsx filter=lfs diff=lfs merge=lfs -text
+脓毒症/转录组/geo_GSE311976/GSE311976_2_transcripts_fpkm_expression.xlsx filter=lfs diff=lfs merge=lfs -text
+脓毒症/转录组/geo_GSE311976/GSE311976_gene_count_matrix.xlsx filter=lfs diff=lfs merge=lfs -text
+脓毒症/转录组/geo_GSE311976/GSE311976_transcript_count_matrix.xlsx filter=lfs diff=lfs merge=lfs -text

脓毒症/转录组/geo_GSE311976/GSE311976_1_genes_fpkm_expression.xlsx

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脓毒症/转录组/geo_GSE311976/card.md

@@ -0,0 +1,38 @@
+# GSE311976 — Piezo1 Knockdown Activates PI3K/AKT and Enhances SPP1 to Drive M2 Macrophage Polarization and Reduce Cardiac Inflammation
+
+> 脓毒症(Sepsis)相关数据集 · 信息卡 · 由自动采集流水线生成 2026-06-20 09:45:49 UTC
+
+## 基本信息
+
+| 字段 | 内容 |
+|---|---|
+| 登记号 Accession | `GSE311976` |
+| 数据源 Source | GEO |
+| 数据类型 Type | 转录组 (transcriptome) |
+| 物种 Organism | Mus musculus |
+| 样本数 Samples/Cells | 6 |
+| 年份 Year | 2025 |
+| 原始页面 Landing | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE311976 |
+| 下载层级 Level | 处理数据+元数据 |
+| 本地下载大小 | 32.79 MB |
+
+## 摘要 Summary
+
+We have successfully established a mouse model with myeloid cell-specific knockdown of Piezo1.   The intraperitoneal injection of lipopolysaccharide (LPS) resulted in a significant increase in cardiac macrophage infiltration, as well as an increase in the expression of inflammatory factors and the inflammatory response.   However, myeloid cell-specific knockdown of Piezo1 impaired this response, leading to an increase in macrophage polarization towards the M2 type and the decreased inflammatory response.   As a result, myocardial injury caused by sepsis was attenuated.   We have also demonstrated that the PI3K/AKT pathway is significantly activated after Piezo1 knockdown, resulting in reduced myocardial dysfunction.
+
+## 文件 Files
+
+| 文件 | 大小(MB) | 链接/备注 |
+|---|---|---|
+| `GSE311976_series_matrix.txt.gz` | 0.003 | https://ftp.ncbi.nlm.nih.gov/geo/series/GSE311nnn/GSE311976/matrix/GSE311976_series_matrix.txt.gz |
+| `GSE311976_1_genes_fpkm_expression.xlsx` | 9.014 | https://ftp.ncbi.nlm.nih.gov/geo/series/GSE311nnn/GSE311976/suppl/GSE311976_1_genes_fpkm_expression.xlsx |
+| `GSE311976_2_transcripts_fpkm_expression.xlsx` | 17.564 | https://ftp.ncbi.nlm.nih.gov/geo/series/GSE311nnn/GSE311976/suppl/GSE311976_2_transcripts_fpkm_expression.xlsx |
+| `GSE311976_gene_count_matrix.xlsx` | 1.758 | https://ftp.ncbi.nlm.nih.gov/geo/series/GSE311nnn/GSE311976/suppl/GSE311976_gene_count_matrix.xlsx |
+| `GSE311976_transcript_count_matrix.xlsx` | 4.449 | https://ftp.ncbi.nlm.nih.gov/geo/series/GSE311nnn/GSE311976/suppl/GSE311976_transcript_count_matrix.xlsx |
+
+## 后续分析建议 Analysis hints
+
+DESeq2/limma 差异表达 → GSEA/GO/KEGG 富集。
+
+---
+*脓毒症数据采集流水线 · Hugging Face 镜像 · 数据版权归原始提交者，使用请遵循各库许可与引用要求。*

脓毒症/转录组/geo_GSE311976/metadata.json

@@ -0,0 +1,40 @@
+{
+  "source": "geo",
+  "accession": "GSE311976",
+  "data_type": "transcriptome",
+  "title": "Piezo1 Knockdown Activates PI3K/AKT and Enhances SPP1 to Drive M2 Macrophage Polarization and Reduce Cardiac Inflammation",
+  "organism": "Mus musculus",
+  "n_samples": 6,
+  "year": "2025",
+  "summary": "We have successfully established a mouse model with myeloid cell-specific knockdown of Piezo1.   The intraperitoneal injection of lipopolysaccharide (LPS) resulted in a significant increase in cardiac macrophage infiltration, as well as an increase in the expression of inflammatory factors and the inflammatory response.   However, myeloid cell-specific knockdown of Piezo1 impaired this response, leading to an increase in macrophage polarization towards the M2 type and the decreased inflammatory response.   As a result, myocardial injury caused by sepsis was attenuated.   We have also demonstrated that the PI3K/AKT pathway is significantly activated after Piezo1 knockdown, resulting in reduced myocardial dysfunction.",
+  "landing_url": "https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE311976",
+  "files": [
+    {
+      "name": "GSE311976_series_matrix.txt.gz",
+      "url": "https://ftp.ncbi.nlm.nih.gov/geo/series/GSE311nnn/GSE311976/matrix/GSE311976_series_matrix.txt.gz",
+      "size_mb": 0.003
+    },
+    {
+      "name": "GSE311976_1_genes_fpkm_expression.xlsx",
+      "url": "https://ftp.ncbi.nlm.nih.gov/geo/series/GSE311nnn/GSE311976/suppl/GSE311976_1_genes_fpkm_expression.xlsx",
+      "size_mb": 9.014
+    },
+    {
+      "name": "GSE311976_2_transcripts_fpkm_expression.xlsx",
+      "url": "https://ftp.ncbi.nlm.nih.gov/geo/series/GSE311nnn/GSE311976/suppl/GSE311976_2_transcripts_fpkm_expression.xlsx",
+      "size_mb": 17.564
+    },
+    {
+      "name": "GSE311976_gene_count_matrix.xlsx",
+      "url": "https://ftp.ncbi.nlm.nih.gov/geo/series/GSE311nnn/GSE311976/suppl/GSE311976_gene_count_matrix.xlsx",
+      "size_mb": 1.758
+    },
+    {
+      "name": "GSE311976_transcript_count_matrix.xlsx",
+      "url": "https://ftp.ncbi.nlm.nih.gov/geo/series/GSE311nnn/GSE311976/suppl/GSE311976_transcript_count_matrix.xlsx",
+      "size_mb": 4.449
+    }
+  ],
+  "level": "处理数据+元数据",
+  "harvested_utc": "2026-06-20 09:45:49"
+}